High-Temperature L-Lactic Acid Fermentation of Food Waste Under Open Condition and Its FISH Analysis of Its Micro Flora
نویسندگان
چکیده
When minced model kitchen refuse was inoculated with Bacillus coagulans and incubated under non-sterilizing open condition, at the temperature below 45°C, racemic mixture of D-and L-lactic acid was found, while L-lactic acid was selectively accumulated at 50-65°C. To analyze microflora of such mixed culture system, whole cells (fluorescent in situ hybridization) using species and group of specific 16S rRNA-targeted oligonucleotide probes (16S-FISH) was applied. As a result, predominancy of mesophilic lactic acid bacteria at lower temperature, and of Bacillus coagulans at higher temperature was confirmed.
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